Inauguration of Open RT-PCR Laboratory - A Defining Moment in the life of BCMCH

Until yesterday, we were using closed qPCR* (quantitative real-time PCR) / RT-qPCR (reverse transcription–qPCR) systems like Truenat from Molbio Diagnostics and GeneXpert from Cepheid. These facilities are good. Using these systems, we can perform tests only with the reagents and kits provided by the company. Also, these systems offer only low throughput (that means the cumulative time taken to process bulk samples is usually longer). 

Now, with the Open qPCR/RTqPCR system, inaugurated on 6 October 2020, we can perform bulk samples at the same time, thereby providing high through output. This facility offers flexibility to use any qPCR/RT-qPCR kits and consumables from various manufacturers and also helps us develop in-house PCR assays using the open qPCR/RTqPCR platform. Diagnostic applications of qPCR/RT-qPCR includes quantification and detection of the genetic material of disease-causing microorganisms, identification of serotypes and genotypes, detection of human DNA sequence variants and its association with various ailments including cancer. Open qPCR also helps to carry out research in single nucleotide polymorphisms (SNPs), gene expression analysis, MicroRNA profiling, Pharmacogenomics, and telomere length analysis and characterization of the gut microbiome.

(Reference ** The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments )

Yesterday’s dedication was a defining moment in the life of BCMCH.

We now have a MOLECULAR BIOLOGY Laboratory, which provides us with unlimited possibilities in the field of research.

We thank God for making all this possible

We have been accredited by ICMR and NABL.